MovDisordV3, Main, Exploration, bibRecord, 004B83

Isolation and 13C-NMR characterization of an insoluble proteinaceous fraction from substantia nigra of patients with Parkinson's disease

Identifieur interne : 004B83 ( Main/Exploration ); précédent : 004B82; suivant : 004B84

Isolation and 13C-NMR characterization of an insoluble proteinaceous fraction from substantia nigra of patients with Parkinson's disease

Auteurs : Silvio Aime [Italie] ; Bruno Bergamasco [Italie] ; Mariano Casu [Italie] ; Giuseppe Digilio [Italie] ; Mauro Fasano [Italie] ; Sabrina Giraudo [Italie] ; Leonardo Lopiano [Italie]

Source :

Movement disorders [ 0885-3185 ] ; 2000.

RBID : Pascal:00-0479900

Descripteurs français

Pascal (Inist)
- Parkinson maladie, Locus niger, Pigment organique, Spectrométrie RMN, Résonance paramagnétique électronique, Caractéristique, Exploration, Pathogénie, Personne âgée, Neuromélanine.
Wicri :
- topic : Personne âgée.

English descriptors

KwdEn :
- Characteristic, Elderly, Electron paramagnetic resonance, Exploration, Locus niger, NMR spectrometry, Organic pigment, Parkinson disease, Pathogenesis.

Abstract

Neuromelanin is a dark brown pigment suspected of being involved in the pathogenesis of Parkinson's disease. This pigment can be isolated from normal human substantia nigra by a procedure that includes an extensive proteolytic treatment. In this study we used such a procedure to extract the neuromelanin pigment from a pool of substantia nigra from patients affected by Parkinson's disease. ¹³C Cross polarization magic angle spinning nuclear magnetic resonance spectroscopy and electron paramagnetic resonance spectroscopy were used to characterize the solid residue obtained from the extraction procedure. We found that the pigment extracted from the substantia nigra of parkinsonian patients was mainly composed of highly cross-linked, protease-resistant, lipo-proteic material, whereas the neuromelanin macromolecule appears to be only a minor component of this extract. A synthetic model of melanoprotein has been prepared by enzymatic oxidation of dopamine in the presence of albumin. Once it has undergone the same proteolytic treatment, this model system yields a ¹³ C-NMR spectrum which is similar to that observed for the parkinsonian midbrain extract. These results are consistent with the view that oxidative stress has a relevant role in the pathogenesis of Parkinson's disease.

Affiliations:

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Le document en format XML

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C-NMR characterization of an insoluble proteinaceous fraction from substantia nigra of patients with Parkinson's disease</title>
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C-NMR characterization of an insoluble proteinaceous fraction from substantia nigra of patients with Parkinson's disease</title>
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<front><div type="abstract" xml:lang="en">Neuromelanin is a dark brown pigment suspected of being involved in the pathogenesis of Parkinson's disease. This pigment can be isolated from normal human substantia nigra by a procedure that includes an extensive proteolytic treatment. In this study we used such a procedure to extract the neuromelanin pigment from a pool of substantia nigra from patients affected by Parkinson's disease. <sup>13</sup>
C Cross polarization magic angle spinning nuclear magnetic resonance spectroscopy and electron paramagnetic resonance spectroscopy were used to characterize the solid residue obtained from the extraction procedure. We found that the pigment extracted from the substantia nigra of parkinsonian patients was mainly composed of highly cross-linked, protease-resistant, lipo-proteic material, whereas the neuromelanin macromolecule appears to be only a minor component of this extract. A synthetic model of melanoprotein has been prepared by enzymatic oxidation of dopamine in the presence of albumin. Once it has undergone the same proteolytic treatment, this model system yields a <sup>13</sup>
 C-NMR spectrum which is similar to that observed for the parkinsonian midbrain extract. These results are consistent with the view that oxidative stress has a relevant role in the pathogenesis of Parkinson's disease.</div>
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	Movement Disorders (revue)
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Movement Disorders (revue)

Isolation and 13C-NMR characterization of an insoluble proteinaceous fraction from substantia nigra of patients with Parkinson's disease

Isolation and 13C-NMR characterization of an insoluble proteinaceous fraction from substantia nigra of patients with Parkinson's disease

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri